Expression analysis of cytochrome p450 genes in pyrethroid-resistant Triatoma infestans

MORICONI, DÉBORA E.; SALAMANCA-MORENO, JHON A.; CALDERÓN FERNÁNDEZ, GUSTAVO M.; DULBECCO, ANDREA B.; PEDRINI, NICOLÁS; JUAREZ, M.PATRICIA

Córdoba

Congreso; LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2016

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

The blood-sucking bug Triatoma infestans (Hemiptera, Reduviidae) is the most widespread and relevant vector of Chagas disease in the southern region of South America. Although chemical insecticides have been a successful vector control strategy, a growing number of deltamethrin resistance foci are being documented. Among genes involved in detoxification processes in insects, cytochrome P450 monooxygenases (CYP) are often associated with relevant roles in metabolic resistance. Using the Rhodnius prolixusgenome information available, we characterized 33 CYP genes belonging to clans 2, 3, 4, and mitochondrial in the integument of T. infestans. Five genes belonging to clan 3 and four genes from clan 4 were up-regulated in deltamethrin-resistant fifth-instar nymphs compared with deltamethrin-susceptible specimen, as measured by qPCR. These results suggest that induction of specific CYP genes might be responsible, at least in part, for the high levels of deltamethrin resistance detected in T. infestans.