Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data

ALMEIDA, F; HURTADO, JP; HASSON, E; REVALE, S; ALMEIDA, F; HURTADO, JP; HASSON, E; REVALE, S

Arthropod Systematics & Phylogeny

Senckenberg Gesellschaft für Naturforschung

Lugar: Dresden; Año: 2019 vol. 77 p. 239 - 250

1863-7221

The Drosophila buzzatii cluster is a South American clade thatencompasses seven closely related cactophilic species and constitutes avaluable model system for evolutionary research. Though the monophyly of thecluster is strongly supported by molecular, cytological and morphologicalevidence, phylogenetic relationships within it are still controversial. Thephylogeny of the D. buzzatii clusterhas been addressed using limited sets of molecular markers, namely a fewnuclear and mitochondrial genes, and the sharing of fixed chromosomalinversions. However, analyses based on these data revealed inconsistenciesacross markers and resulted in poorly resolved basal branches. Here, we revisethe phylogeny of the D. buzzatiicluster based on a large transcriptomic dataset of 813 kb obtained from fourmembers of this cluster: D. antonietae,D. borborema, D. buzzatii and D. koepferae,using the close relative D. mojavensis(also a member of the repleta group)as outgroup. Our phylogenomic analyses confirm that D.buzzatii is sister to the other six members of the cluster and, thoughincomplete lineage sorting likely obstructs phylogenetic resolution among thesesix species, allowed us to recover a novel topology. Divergence timeestimates date the radiation of the cluster to the recent upper Pleistocenewith most speciation events compressed to the last 500,000 years.<!-- /* Font Definitions */ @font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:roman;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin:0in;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:"Times New Roman",serif;mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:EN-US;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-US;mso-fareast-language:EN-US;}@page WordSection1{size:8.5in 11.0in;margin:70.85pt 85.05pt 70.85pt 85.05pt;mso-header-margin:.5in;mso-footer-margin:.5in;mso-paper-source:0;}div.WordSection1{page:WordSection1;}-->