The T98G glioblastoma as a cell model to investigate the circadian clock and phospholipid metabolism function in human tumor cells

LUCAS G. SOSA ALDERETE,; NATALIA MONJES; IGNACIO CHEVALLIER-BOUTELL ; MARIO E. GUIDO.

Congreso; L Reunión Anual de la Sociedad Argentina de investigaciones en Bioquímica y Biología Molecular; 2014

The circadian system temporarily regulates various behavioral, physiological and metabolic processes and is comprised of central and peripheral oscillators distributed in various organs, tissues and even in immortalized cell lines. Studies in animals and epidemiological evidence suggests that disruption of circadian rhythms by environmental and genetic effects can lead to metabolic diseases such as hyperlipidemia, fat liver, obesity and high incidence of developing cancer. The objectives of this study were to evaluate whether an immortalized cell line from human tumor such as T98G retains functional the machinery of temporal expression of clock genes such as Bmal1, Per1 and a clock controlled gene (CCG) like CK alpha1 as well as analyze whether the total phospholipids (FLs) biosynthesis has temporal profiles. To them, the T98G was grown in DMEM supplemented with 10% FBS for 48-72h. After synchronization with dexamethasone (100 nM), T98G cell were maintained under proliferative conditions (P) or partial arrest (AP) for 48 h. The results showed that the clock genes Bmal1, Per1 and the CCG, CK alpha1, conserved a temporal expression under both growth conditions (P and AP), with a period of 24-28 h. Similarly, in the FLs labeling, it was found that the T98G cell displayed a circadian fashion in the PLs metabolism with a period of 20 h and reached the highest levels at 20 h and 48 h under both culture conditions. These results suggest that these tumor cells under the experimental conditions studied preserve the temporal control of the molecular clock and the biosynthesis of total PLs.