Soluble guanylyl cyclase alpha1 subunit mediates cell proliferation, survival and migration in human cervical tumor cell line HeLa

RONCHETTI SONIA; BEATRIZ DUVILANSKI; MARIA TERESA LUJAN PINO; CORDEIRO GEORGINA; BOLLANI SABRINA; CABILLA JIMENA

Mar del Plata

Congreso; LVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2018

Soluble guanylyl cyclase is an heterodimeric enzyme composed by two subunits, alpha1 (a1) and beta1 (b1). Previously we have shown that a1 increased levels strongly correlate with E2-induced proliferation in E2-depentent tissues and a1 knock-down decreased cell proliferation in estrogen-responsive endometrial and breast cancer cell lines. a1 role in tumor progression is widely unknown, however it was shown that a1 blocked p53 in some tumor prostate cell lines. The aim of the present study was to investigate the role of a1 in proliferation, survival and migration in the E2-unresponsive, p53-defective cervical tumor cell line HeLa.a1 expression was silenced through siRNA specific sequences using scramble sequences as control. Protein levels were measured by western blot. Apoptosis and mitosis were assessed by nuclear morphology (Hoechst). Apoptosis were measured by Annexin-V/PI staining. Cell cycle was studied by flow cytometry. Migration was determined through scratch motility and transwell assays. a1 knock-down (a1KD) significantly reduced PCNA expression (20% decrease vs control, p