Comparative analysis of spermatogenesis and hormonal profile of infertile patients with idiopathic orchitis versus rats with autoimmune orchitis

AMARILLA MARÍA SOFÍA; SOBARZO CRISTIAN MARCELO,; THEAS MARIA SUSANA; JACOBO PV,; MAIO MARIA BELEN; GLIENKE L; LUSTIG, LIVIA

Ciudad Autonoma de Buenos Aires

Congreso; REUNION ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACION CLINICA; 2021

SOCIEDAD ARGENTINA DE INVESTIGACION CLINICA

Experimental autoimmune orchitis (EAO) is a well-established rodent model of organ specific autoimmunity associated to infertility. Testis immunopathology is similar in rats and humans undergoing a chronic testicular inflammation. A comparative analysis of other aspects of the disease like the quantification of spermatogonia (Spg) and Sertoli cells (SCs), by immunohistochemistry, as well as the hormonal serum profile of infertile patients with idiopathic orchitis vs rats with EAO was undertaken (RIA). We evaluated testicular biopsies from patients with idiopathic non-obstructive azoospermia, diagnosed with hypospermatogenesis (mild: n=8, severe: n=10) (HypE) and Sertoli cell only syndrome (SCOS, n=9). All groups displayed twice the number of immune cells (CD45+) vs patients with obstructive azoospermia and complete spermatogenesis (control group, C, n=8). The number of undifferentiated and differentiated SPg/seminiferous tubule (ST) decreases in mild and severe HypE while the number of SCs/STs increases in severe HypE and SCOS vs. control (p