Parenteral Succinate Reduces Systemic ROS Production in Septic Rats, but It Does Not Reduce Creatinine Levels

SEBASTIÁN P. CHAPELA; ROMINA CANZONIERI; CARLOS STELLA; HILDA BURGOS; ALEXIS MURYAN; CHRISTIAN CONGOST; MANUEL ALONSO

OXIDATIVE MEDICINE AND CELLULAR LONGEVITY

LANDES BIOSCIENCE

Lugar: Austin, Texas; Año: 2018 vol. 2018 p. 1 - 6

1942-0900

Copyright © 2018 Sebastián P. Chapela et al. This is an open access article distributed under the Creative Commons AttributionLicense, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work isproperly cited.In sepsis, reactive oxygen species (ROS) production is increased. This process takes place mainly within the electron transportchain. ROS production is part of the pathophysiology of multiple organ failure in sepsis. Succinate yields Dihydroflavine-Adenine Dinucleotide (FADH2), which enters the chain through complex II, avoiding complex I, through which electrons arelost. The aim of this work is to determine if parenteral succinate reduces systemic ROS production and improves kidneyfunction. Rats with cecal ligation and puncture were used as model of sepsis, and 4 groups were made: Control group; Succinategroup, which only received parenteral succinate; Sepsis group; and Sepsis which received parenteral succinate. Systemic ROS aremeasured 24 hours after the procedure. Rats subjected to cecal puncture treated with succinate had less systemic ROS thanSeptic untreated rats (p = 0 007), while there were no differences in creatinine levels (p = 0 07). There was no correlationbetween creatinine and systemic ROS levels (p = 0 3). We concluded that parenteral succinate reduces ROS levels, but it doesnot reduce creatinine levels. Since there is no correlation between both levels, the processes would not be related.