Association between Aggregatibacter Actinomycetemcomitans and Porphyromonas Gingivalis in subgingival plaque and clinical parameters, in argentine patients with aggressive periodontitis

SÁNCHEZ, G.A.; ACQUIER, A.; DE COUTO PITA, A.K.; BUSCH, L.; MENDEZ, C.F.

MICROBIAL PATHOGENESIS

ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2015 vol. 82 p. 31 - 36

0882-4010

Background: Aggregatibacter actinomycetemcomitans (Aa) andPorphyromonas gingivalis (Pg) have been associated with aggressive (AgP)and chronic periodontitis.Objective: The aim of this study was to evaluate the levels of Aa and Pg ingingival crevicular fluid (GCF) of patients with AgP and its relation with clinicalparameters.Design: Sixteen females and fourteen males with clinical diagnosis of AgPaged 17-23 years and their match?s controls, were included in this study.Clinical recording concerning probing pocket depth, clinical attachment level,plaque index and gingival bleeding index were performed at baseline, 30 and 60days after baseline. After clinical examination GCF samples were analyzed forAa and Pg with a real-time polymerase chain reaction technique. Patients groupwas treated with a combined of mechanical and oral antibiotic therapy(doxycycline 100 mg/ day, during 21 days). A multivariate analysis was used todetermine the relationship between Aa and Pg counts with clinical parameters.Results: GCF from all subjects was positive for Aa and PG. In controls Pgconcentration was higher than Aa (Pg: 42420 ± 3034 copies/ml; Aa: 66.6 ± 5.4copies/ ml p< 0.001) while in patients both microbes showed the sameconcentration (Aa: 559878 ± 39698 Pg: 572321 ± 58752). A significant andpositive correlation was observed between counts of Aa and Pg (R square:0.7965, p<0.0001). Female showed more counts/ ml. Aa might be closelyassociated with clinical parameters while Pg did not. At 30 and 60 days Aacounts in patients were similar to controls while Pg counts were equal tobaseline. However, in spite of Pg presence a clinical improvement wasobserved in all patients.Conclusions: In our population the presence of Aa may be associated withAgP while Pg may be in GCF as an opportunistic pathogen which might causeddisease when the ecological balance was favourable.