Iron deficiency induced response in Arabidopsis thaliana roots: Role of ethylene

CABALLERO VERONICA; D'IPPOLITO SEBASTIAN; MASSAZZA DIEGO; NEGRI PEDRO; RAMÍREZ LEONOR; SCHROTT GERMAN; TAGLIOTTI MARTIN; PAGNUSSAT LUCIANA; PINEDO MARCELA; LAMATTINA LORENZO

Rosario, Santa Fe

Congreso; SAIB; 2006

SAIB

A low availability of iron in the ground generates a series of responses in plants roots leading to the increase iron uptake. Among them are the induction of expression and activity of an Fe+3 reductase (fro2), increment in the expression of a transcription factor (fit1) and morphologic changes in roots. There are evidences of nitric oxide (NO) and ethylene participation in iron deficiency induced response in plant roots. The objective of this research was to study the role of ethylene during iron deficiency in Arabidopsis thaliana. Plants grown in presence or absence of iron, with etephon (ethylene donor) or Cl2Co (ethylene synthesis inhibitor) additions were used. The fro2 and fit1 expression, evaluated by RT PCR indicated that Cl2Co did not affect the mRNA levels in presence or absence of iron. The morphologic analysis of the roots demonstrated that depletion of endogenous ethylene inhibits roots hairs development, in contrast to the observed in iron deficiency. NO production in roots incremented in presence of the ethylene addition, whereas it decreases when adding of Cl2Co. As a conclusion, ethylene would play an essential role in root hairs development and NO production, but ethylene would not be involved in fro2 and fit1 expression, nor in iron reductase activity.