Differential expression of PI-PLCs in tomato cells exposed to salt stress.

RAMIREZ LEONOR; TEN HAVE ARJEN; LAMATTINA LORENZO; LAXALT ANA

Mar del Plata, Buenos Aires

Congreso; SAIB; 2007

SAIB

Salt stress is known to generate phosphatidic acid via the concerted action of phosphoinositide-specific phospholipases C (PI-PLCs) and 1,2-diacylglycerol kinases. Both PI-PLC and DGK are encoded by large gene families. We are interested in elucidating the gene-specific expression profiles of individual PI-PLC during salt stress in tomato. In Solanum lycopersicum six PI-PLCs genes have been identified so far. We studied the expression patterns of PI-PLCs by RT-PCR in tomato suspension cells exposed to different doses of NaCl and KCl. The results showed a differential mode of expression of each PLC depending on the doses and the duration of the treatment. A property of many signaling enzymes is that treatments that activate them often rapidly enhance expression of their genes. Thus, those which are up-regulated are good candidates for being involved in salt stress signaling. Our laboratory showed that treatments with salt stress induced Nitric Oxide (NO) production and it activates PLC signaling in tomato cells, future analysis will be directed to unravel the role of NO during salt activated PI-PLCs.