Impact of bariatric surgery on absolute telomere length and mitochondrial DNA mass in obese patients

ANDREA L MILLÁN; AXEL BESKOW; GUSTAVO DANIEL FRECHTEL; MAILÉN ROJO; GUTT SUSANA; MARIA CONSTANZA PAUTASSO; GLORIA EDITH CERRONE

Mar del Plata

Congreso; Reunión Anual de SOCIEDADES DE BIOCIENCIA; 2022

SAIC

Objective: The main objective of the present study was to evaluate the change in the mass of mitochondrial DNA (mtDNA) and the absolute leukocyte telomere length (LTLa), in patients with obesity before and after 6 months of the bariatric surgery. The secondary objective was to evaluate the probable association of the change in these markers, with the expression of key molecules of inflammation such as TLR4 and IL1-𝛽Methods: 37 individuals with an indication for bariatric surgery were included. LTLa was determined in leukocytes using qPCR through standard curves by the T/S ratio. mtDNA mass, TLR4 and IL1b expression were determined by the 2-ΔΔCt method. We evaluated the change of variables by analysis of related samples and correlations and linear regressions between variables by SPSS V20Results: After surgery patients showed a significant weight loss and an improvement in the metabolic lipid profile, accompanied by a decrease in inflammation determined by hs-CRP. The change in visceral fat correlated significantly with changes in blood glucose (r=0.391; p=0.020), HbA1c (r=0.448; p=0.013) and HOMA index (r=0.548; p=0.022). We did not observe changes in the expression of TLR4 and IL1-𝛽, although the reduction in blood glucose levels and HbA1c associated with the lower expression of IL1-𝛽 (p=0,042 y p=0,048 respectively). Finally, the patients showed an increase of LTLa (p=0,004) and higher mtDNA mass (p=0.015) after surgery. The increase in LTLa mass was correlated to the decrease in hs-CRP (r=-0.71; p