THE IntIPstQ-like INTEGRASE RECOMBINATION SITES (attCSITES)ARE POORLY ASSOCIATED TO attCsFROM ANTIMICROBIAL RESISTANCE GENE CASSETTES OF CLASS 1 INTEGRONS

QUIROGA MARIA PAULA; ALONSO FERNANDO; ALVAREZ VERÓNICA ELIZABETH; ALVAREZ VERÓNICA ELIZABETH; CENTRÓN DANIELA; GAMBINO ANAHÍ; CENTRÓN DANIELA; GAMBINO ANAHÍ; QUIROGA MARIA PAULA; ALONSO FERNANDO

Buenos Aires

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Sociedades Argentinas de BioCiencias

Pseudomonasstutzeri is a naturally competent gram-negative specieswidely distributed in the environment, also described as an opportunisticpathogen and integron carrier. Such characteristics make P. stutzeri a promising organism for the study of lateral genetictransfer (LGT) mechanisms. In order to study thediversity of the attC recombinationsites associated with IntIPstQ-like and its contribution to multidrug resistantintegrons we performed a search for sequences with an identity >65%with intIPstQ integrase. Sequences wereanalyzed with INTEGRON FINDER to detect: C-In (complete integrons, Integrase+attC), CALINs (clusters of attC sites lacking integron-integrases)and In0 (Integrase only). Sequenceand phylogenetic analysis of intIPstQwere made to establish its relationship with other integron integrases. We alsocarried out a phylogenetic analysis of the obtained attC sites and the ones of the main antibiotic resistance genecassettes (ARGC). Twenty sequences belonging to Pseudomonas, Halothiobacillus,Azotobacter, Methylibium, Thioalkalivibrio were analyzed in which 15 C-In, 25 CALINs and 4In0 were identified. IntIPstQ was found more related to IntI2, evidencing the tetrad RHRYconserved in most cases, and 50% of the strains showed an ALAR motif. A total of 285 attC sites were found, 170 located in C-In and 115 in CALINs. The length of attCsites was between 42 and 132bp, being the most frequent of 76bp. Most attC sites from ARGCs were found in 3main subclusters related to attCsfrom Halothiobacillus sp., Azotobacter and Methylibiumwhile only attCqnrVC1 was identifiedamong attC sites from Pseudomonas. Taken together, our resultssuggest that the genus Pseudomonas isnot an active reservoir of attC sitesthat circulate by the mechanisms of the LGT in the genetic platform of class 1integrons, although IntIPstQ-related integrases with attCs sites belonging to other clustersare part of the resistome of ARGCs