A multidrug resistant Klebsiella oxytoca ST2 strain harboring a blaKPC-2 -plasmid disseminates amongst colonized patients

KNECHT CAMILA; ALVAREZ VERÓNICA ELIZABETH; GONZALEZ MACHUCA ADRIÁN; CAMPOS JOSEFINA; AYALA NÚÑEZ T; DONIS NICOLÁS; QUIROGA MARIA PAULA; LEGUINA CAROLINA; GARCÍA ALLENDE NATALIA; MASSÓ MARIANA; PIEKAR MARIA; CAMICIA GABRIELA; ALONSO FERNANDO; CENTRÓN DANIELA; GAMBINO ANAHÍ; PRACK MC CORMICK BARBARA; CARRERA PÁEZ LAURA; FOX BARBARA; VARGAS CAROLINA; FERNÁNDEZ-CANIGIA LILIANA

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Congreso; IUMS Congress, the online edition; 2022

Withinthe genus Klebsiella, Klebsiella oxytoca species Complex(KOSC) is the second most found in hospitals after K. pneumoniae. It has been shown that KOSC can exchangeantimicrobial resistance genes (ARGs) with other bacteria, even class A and Bcarbapenemases, leading to hospital outbreaks. Surveillance, diagnosis andtreatment of carbapenem resistant Enterobacteralesbelong to the highest priority group of the World Health Organization. The aimof this work was to determine ARGs, genetic platforms and plasmids in threecarbapenem resistant K. oxytoca colonizingisolates obtained from three inpatients during hospitalization from ourhospital. Whole-Genome sequencing was performed using Illumina MiSeq-I. Genomicassembly and annotation were done using SPAdes and RAST, respectively. Insilico detection of multi locus sequence typing, core genomes? SNPs, plasmids,ARGs and their genetic platforms was carried out using pubMLST, Snippy-core,PlasmidFinder, resFinder, BLASTn, etc. The three isolates were identified as a singleK. oxytoca sequence type ST2 strain,from the international clonal complex 2 (CC2) with the same multiple acquiredARGs including blaKPC-2gene which was part of a Tn3-like element. The three isolates also sharedpredicted plasmids that belonged to IncFII(K) with the blaKPC-2 gene, Col(pHAD28) and IncM1 incompatibilitygroups. Our results evidence the presence of K. oxytoca ST2 in Argentina and not only the global spread ofmultidrug resistant KPC-producing KOSC CC2 but also the silent dissemination ofthis lineage amongst inpatients that highlight the importance of continuoussurveillance programs to identify blaKPC-2reservoirs as well as prevent eventual outbreaks due to KPC-producingstrains.