EFFECT OF HEXACHLOROBENZENE (HCB) AND PROTEIN X OF THE HEPATITIS B VIRUS (HBV) ON LIVER CELL GROWTH DYSREGULATION

RIDRUEJO, EZEQUIEL ; MIRET, NOELIA; COLI, LUCÍA; GENTILE, EMILIANO; OUBIÑA, JOSÉ; MATHEL, VERÓNICA; KURTZ, MELISA L; ALVAREZ, LAURA

- - Se desarrolló en forma virtual debido a la pandemia de COVID-19

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021 LXVI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2021

SAIC- SAI- AAFE- NANOMED.ar 2021

Chronic hepatitis B and exposure to persistent organic pollutants (POPs) can lead to cellular hepatocarcinoma (HCC), the most common liver tumor. HBV DNA encodes transactivator X (HBx). HCB is a COP promoter of hepatic preneoplastic foci. We have shown that HCB deregulates cell growth in rat liver and HepG2 cells, involving TGF-β1, a reversed effect with an Rβ1 inhibitor.Objectives: To analyze in vitro 2 models of HCC generation -associated to HCB or to the expression of HBx- and in vivo the hepatic and angiogenic promoter HCB effect in Balb/c nude mice inoculatedwith HepG2. M&M: the HCB effect on PCNA (Western blot)/TGF-β1 (RT-PCR) was studied in vitro in: 1.1) Huh-7: HCB at various doses (0.005, 0.05, 0.5 and 5 μM ) and times (15, 30, 60, 90 and 120 min); 1.2) Huh-7 transiently transfected with HBx; 2) HepG2.2.15 (with stable expression of HBV) and 3) EA-hy926 (endothelial). In 1.2, 2 and 3 5 μM HCB, 24h was used. Mice: HCB i.p. (0.3 and 3 mg/ kg), and were inoculated with HepG2. Were evaluated: a) PCNA, b) TGF-β1, c) No. of tumor areas, d) histology (H&E) and e) No. of vessels/mm2. Results: In Huh-7, TGF-β1 increased (20%, p