IMPAM   23988
INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Unidad Ejecutora - UE
artículos
Título:
Antifungal susceptibility profile and molecular identification of Cyp51C mutations in clinical and environmental isolates of Aspergillus flavus from Argentina
Autor/es:
HERMIDA-ALAVA, KATHERINE; GORDÓ, MANUELA; LUQUE, ALICIA; HERMIDA-ALAVA, KATHERINE; GORDÓ, MANUELA; LUQUE, ALICIA; SAUTUA, FRANCISCO; FORMENTO, NORMA; CUESTAS, MARÍA L.; SAUTUA, FRANCISCO; FORMENTO, NORMA; CUESTAS, MARÍA L.; BRITO DEVOTO, TOMÁS; SCANDIANI, MERCEDES; CARMONA, MARCELO; BRITO DEVOTO, TOMÁS; SCANDIANI, MERCEDES; CARMONA, MARCELO
Revista:
MYCOSES
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2020
ISSN:
0933-7407
Resumen:
AbstractBackgroundThe emergence of azole resistance in non‐fumigatus Aspergillus strains is on the raise.ObjectivesTo study the susceptibility profiles and the molecular mechanisms of azole resistance of environmental and clinical strains of Aspergillus flavus from Argentina.MethodsThirty‐five A flavus isolates (18 from soybean seeds and chickpea seeds and 17 from the clinic) were analysed for amphotericin B and azole resistance using the standard microbroth dilution method according to CLSI M38‐A2 guidelines. Sequencing analysis of the cyp51 genes was conducted in those isolates displaying high MICs values to itraconazole, voriconazole and/or posaconazole.ResultsAmong the environmental isolates, 33.3% of them showed high MIC values for at least one triazole whereas 23.5% of the clinical isolates displayed high MIC values for amphotericin B. Point mutations in the Cyp51C gene were recorded in most environmental isolates with non‐wild‐type MIC values.ConclusionsSusceptibility differences among environmental A flavus isolates might suggest the possibility of native resistance to certain triazole antifungals used in the clinic. To the best of our knowledge, this is the first report of antifungal screening of environmental strains of A flavus in soybean seeds and chickpea seeds from Argentina that showed increased resistance to voriconazole and itraconazole in comparison to clinical strains.