Activation of iNKT Cells Prevents Salmonella-Enterocolitis and Salmonella-Induced Reactive Arthritis by Downregulating IL-17-Producing γδT Cells

CERQUETTI, MARÍA C.; AYA CASTAÑEDA, MARÍA DEL R.; NOTO LLANA, MARIÁNGELES; GIACOMODONATO, MÓNICA N.; SARNACKI, SEBASTIÁN H.; GIACOMODONATO, MÓNICA N.; SARNACKI, SEBASTIÁN H.; BLANCO, GUILLERMO; MORALES, ANDREA L.; BLANCO, GUILLERMO; MORALES, ANDREA L.; CERQUETTI, MARÍA C.; AYA CASTAÑEDA, MARÍA DEL R.; NOTO LLANA, MARIÁNGELES

Frontiers in Cellular and Infection Microbiology

www.frontiersin.org

Año: 2017 vol. 7

Reactive arthritis(ReA) is an inflammatory condition of the joints that arises following aninfection. Salmonella enterocolitis is one of the most common infectionsleading to ReA. Although the pathogenesis remains unclear, it is known thatIL-17 plays a pivotal role in the development of ReA. IL-17-producers cells aremainly Th17, iNKT, and gdT lymphocytes. It is known that iNKT cells regulatethe development of Th17 lineage. Whether iNKT cells also regulate gdTlymphocytes differentiation is unknown. We found that iNKT cells play aprotective role in ReA. BALB/c Ja18−/− mice suffered a severe Salmonellaenterocolitis, a 3.5-fold increase in IL-17 expression and aggravated inflammationof the synovial membrane. On the other hand, activation of iNKT cells with a-GalCerabrogated IL-17 response to Salmonella enterocolitis and prevented intestinal andjoint tissue damage. Moreover, the anti-inflammatory effect of a-GalCer wasrelated to a drop in the proportion of IL-17-producing gdT lymphocytes(IL17-gdTcells) rather than to a decrease in Th17 cells. In summary, we hereshow that iNKT cells play a protective role against Salmonella-enterocolitisand Salmonella-induced ReA by downregulating IL17-gdTcells.