Effect of novel Bismuth hydroxide presentations on surfaces contaminated with verotoxigenic Escherichia coli

ANALÍA I. ETCHEVERRÍA; RUIZ M. JULIA; COLELLO, ROCÍO; NORA L. PADOLA; CÁCERES EMILIA

Florencia

Simposio; 10th International Symposium on Shiga Toxin Producing Escherichia coli; 2018

Stefano Morabito Chair of the Local Organising Committee

Effect of novel Bismuth hydroxide presentations on surfaces contaminated with verotoxigenic Escherichia coliRuiz J, Cáceres E., Colello R., Etcheverria, A, Padola NLLaboratorio de Inmunoquímica y Biotecnología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET-CIC-UNCPBA, Facultad de Ciencias Veterinarias, UNCPBA, Tandil, ArgentinaIntroductionVerotoxigenic Escherichia coli (VTEC) are an emerging pathogens associated with cases of diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). Contaminated food, cattle or contact with their environment are some of the problems that especially affect to the food industry, the hospital and domestic environment. Because there is no specific treatment against HUS, the prevention measures and control strategies are the key tools to reduce the transmission of this pathogen. Currently Bismuth hydroxide has been widely used as antidiarrheal but little is known about its bactericidal action on contaminated inert surfaces.The aim of this work was to evaluate the effects on the viability of VTEC O157 and non-O157 strains on glass surfaces with two types of novel Bismuth hydroxide presentations, emulsion spray and aerosol.MethodsThree VTEC strains (serotypes O157:H7, O91:H21 and O26:H11) were grown in LB and they were sown with swab on sterile glass plates. The strains were sprayed independently with both presentations of products and the effects were followed by counting CFU/ml on MacConkey agar plates at different times: 4 h and 24 h.ResultsAfter 4 h of incubation at room temperature, following counts were obtained in strains controls (without treatment with Bismuth): 120 CFU/ml (O157:H7), 190 CFU/ml (O91:H21) and 140 CFU/ml (O26:H11). No growth was observed in plates treated with spray, while in those treated with aerosol 80 CFU/ml was observed only in O91:H21 strain. After 24 h, following counts were obtained in strains controls: 2 CFU/ml (O157:H7), 6 CFU/ml (O91:H21) and no growth (O26:H11). No growth was observed in plates treated with both presentations. ConclusionsAll the strains were affected in their growth after to applicate some of the Bismuth presentations, although the spray showed more efficiency than the aerosol at 4 h. The most resistant strain to time and lack of nutrients was O91:H21, which in turn was the less affected after 4 hours with the aerosol form.Bismuth Hydroxide in these novel presentations is promising as disinfectant on inert surfaces.