INVOLVEMENT OF HOMOCYSTEINE AND ADENOSINE IN ERYTHROPOIETIN RESISTANCE IN HUMAN ERYTHROLEUKEMIA CELLS

MALTANERI, ROMINA E.; CHAMORRO, MARÍA EUGENIA; VITTORI, DANIELA C.; SCHIAPPACASSE AGUSTINA; NESSE, ALCIRA B.

Mar del Plata

Congreso; Reunión Conjunta SIAC SAI SAFIS 2018; 2018

SAIC SAI SAFIS

End Stage Renal Disease (ESRD) is associated with the anemia detected in inflammatory conditions, and negative prognosis arises when hiperhomocysteinemia (HHcy) and accumulation of adenosine (Ado) enhance TNF-α cytotoxicity. Previously, we found that the presence of Ado and Hcy increased the sensitivity of undifferentiated erythroleukemia K562 cells to TNF-α-induced apoptosis, which could not be prevented by erythropoietin (Epo). In this study, we investigated whether differentiated cells could be protected by Epo in an inflammatory environment with Hcy and Ado accumulation. Cells were pre-treated with Epo (10 U/mL, 2 h) and differentiated with hemin (C, 30 μM, 48 h). Hcy (500 μM), Ado (250 μM) and TNF-α (T, 30 ng/mL) were added in the last 24 h of differentiation. Contrary to our observations in undifferentiated cells, Epo prevented TNF-α-induced apoptosis (Hoechst staining: C 17.1±0.9; *T 33.9±1.6; *EpoT 25.2±0.5; *EpoHcyAdoT 35.0±1.5,*P