ROLE OF IL-17A ON AUTOPHAGY DURING HUMAN TUBERCULOSIS

AMIANO N.O.; ROLANDELLI, A.; PALMERO D.J.; TATEOSIAN N.L.; CASTELLO F.A.; CASCO, N.; GARCÍA, V.E.; PELLEGRINI, J.M.; MORELLI M.P.; LEVI, A.; COLOMBO, M.I.

Rosario

Congreso; XXIII Congreso Latinoamericano de Microbiología - VIII Reunión de la Sociedad Latinoamericana de Tuberculosis y otras Micobacteriosis (SLAMTB); 2016

An appropriate immune response against Mycobacterium tuberculosis (Mtb) requires Th1 cytokines, but it has been shown that IFN- alone isnot enough to the complete bacterial eradication. Actually, Th17 cells have been associated with Mtb infection. Autophagy is an immuneeffector mechanism against intracellular pathogens, positively modulated by Th1 cytokines, whereas Th2 cytokines show the opposite effect.Thus, the aim of this work was to study the role of IL-17A on autophagy during active human TB. This study has the approval of the EthicsCommittee of the Hospital Dr. Muñiz. Monocytes (Mo) from TB and healthy donors (HD) were infected and cultured with Mtb-H37Rv± IL-17A(10ng/ml, 24h) and autophagy levels were analyzed by Flow cytometry and Immunofluorescence Microscopy against LC3-IIB. We observe thatIL-17A increases autophagy against Mtb-H37Rv in Mo from HD and TB with strong immunity against Mtb (HR-TB) (p