Relevance of the Blastocyst Conditioned Media on immunotolerance: focus on the control of the inflammatory response

E SOCZEWSKI; L GALLINO; E GRASSO; L FERNANDEZ; R RAMHORST; D VOTA; C PÉREZ LEIRÓS

Mar del Plata

Congreso; Reunión Anual SAI; 2016

The decidualization of human endometrial cells involves changes in their secretome increasing the production of immunomodulatory mediators. It is associated with a sterile inflammatory response that should be later controlled to a tolerogenic microenvironment by maternal and bastocyst-derived factors. Here we focus on the production of immunomodulators during the decidualization process and we explored whether human Blastocyst Conditioned Media (BCM) could control the initial inflammatory response. As an in vitro model we used the Human endometrial stromal cell line (HESC) decidualized or not with medroxiprogesterone (10-6M)+dbcAMP (2,5 10-3M) during 8 days. We observed an increase in the production of IDO (indoloamine-2,3 di-oxigenase), CXCL8, CXCL12 and IL-1beta production (p