Annotation and metabolic network construction of the probiotic strain Lactobacillus acidophilus ATCC 4356, visualization in XOMEQ platform

LUCIANNA LEONARDO; BURGUENER GERMÁN; SOSA EZEQUIEL; PALOMINO MARÍA MERCEDES; ALLIEVI MARIANA CLAUDIA; RUZAL SANDRA M; TURJANSKI ADRIÁN; MARTÍ MARCELO; FERNÁNDEZ DO PORTO DARÍO

Bahía Blanca

Congreso; VI Congreso Argentino de Bioinformática y Biología Computacional; 2015

The original Lactobacillus acidophilus strain ATCC 4356 was isolated in 1900 from human infant feces [1]. L. acidophilus ATCC 4356 is an important inhabitant of the gastrointestinaltract (GIT) with reported probiotic properties [2?4]. The genome sequence was obtained using HiSeq 1500 Illumina at Instituto de Agrobiotecnolgía de Rosario (INDEAR), Argentina.Assembly was done with Stampy v1.0.27 [5], using L. acidophilus La-14 as a template. Genomic annotations were assigned automatically by our own Annotation Pipeline [6].Afterwards, we used Pathway tools [7] to build a comprehensive metabolic network (MN) of the bacteria. The software automatically associates genes with reactions, based on enzymecode (EC) numbers and function descriptions contained in the GenBank file of the annotated genome.As a last step in our procedure, the annotated genome was deployed in our server: XOMEQ platform (http://www.biargentina.com.ar/xomeq/).