Correlations between metabolic profiles and lifespan in Saccharomyces cerevisiae cells

ANA LUZ ZUPAN; PALAVECINO MARCOS; BERMUDEZ MORETTI MARIANA; FORFAR ISABELLE; CORREA GARCIA SUSANA

Rosario

Congreso; L Reunion anual de la Sociedad Argentina de Investigacion Bioquimica y Biologia Molecular; 2014

SAIB

In all living organisms, cell survival is mediated by metabolic regulation in response to environmental conditions. S. cerevisiae is used to study the molecular and genetic mechanisms of aging in two distinct ways.The first, replicative life span (RLS), is a measure of the number of mitotic events an individual mother cell can undergo before senescence; the second, chronological life span (CLS), is a measure of the time a non-dividing cell population remains viable. RLS has been suggested to be a model for the aging of mitotic tissues, whereas CLS has been likened to the aging of post-mitotic tissues. Since it is known that both some amino acids and mutations in some amino acid permeases genes can extend CLS, we measured the levels of Uga4 and Agp1 permeases along the aging process. We found a deregulation of their expression in chronologically aged cells. We also analysed the metabolic fingerprints revealed with Fourier Transformed-Infrared (FT-IR) spectroscopy in cells with different lifespan (i.e. cells deficient in TOR or Sch9 kinases). During the aging process, metabolic profiles significantly changed exhibiting important alterations, particularly in the proteins and lipidic peroxidation spectral windows. Moreover, an excellent metabolomics-based discrimination between cells with different lifespan was established.