TISSUE TRANSGLUTAMINASE EXPRESSED IN TROPHOBLAST CELLS AS A POSIBLE TARGET OF AUTOANTIBODIES CONTRIBUTING TO PREGNANCY COMPLICATIONS OF CELIAC PATIENTS

SÓÑORA C; CALO G; FRACCAROLI L; HAUK V; HERNANDEZ A; PEREZ LEIROS C; RAMHORST R

Iguazú

Simposio; V LASRI - Latin American Chapter of the American Society of Reproductive Immunology.; 2013

Background: Celiac disease is associated with pregnancy complications. Antibodies against tissue transglutaminase (tTG) could mediate pathogenic mechanisms since tTG has multiple functions at the maternal-fetal interface. We analized if autoantibodies affect trophoblast cell migration and apoptotic cell phagocytosis by macrophages. Methods: An injury was generated in confluent trophoblast monolayer (Swan-71) and wound healing assessed by microscopy at 4-8-24h in the presence ofMoAb-TG2 (10ug/ml), celiac women sera or normal serum (1:50). Phagocytosis using differentiated THP-1 cells and apoptotic Swan-71cells (1:1) were performed with MoAb-TG2 or women sera. Phagocytosis percentage was determinated by flow citometry CD14+CFSE+ cell counting. Results: Both AcMo-TG2 and celiac women sera delayed injury healing compared to normal sera. AcMo-TG2 and some celiac sera but not normal sera inhibited phagocytosis of apoptotic trophoblast cells.