Chemokines mediated interaction between maternal monocytes and trophoblast cells is differentialy induced by PAMPs stimuli

GRASSO E; D. PAPARINI; FRACCAROLI L; HAUK V; SALAMONE G; C. PÉREZ LEIRÓS; . L. FRACCAROLI, J. ALFIERI, L. LAROCA, V. ROCA, C. PÉREZ LEIRÓS Y R. RAMHORST.L. FRACCAROLI, J. ALFIERI, L. LAROCA, V. ROCA, C. PÉREZ LEIRÓS Y R. RAMHORST

Iguazú

Simposio; V SLIMP - Latin American Society for Maternal Fetal Interaction & Placenta V LASRI - Latin American Chapter of the American Society of Reproductive Immunology.; 2013

Background: We investigated chemokine/chemokine receptor modulation on monocytes during their interaction with trophoblast cells; and whether PAMPs stimuli modulate their functional profile and migratory properties. Methods: Swan 71 cells were cultured with monocytes isolated from fertile women in transwell sytems that allow or not monocyte migration. Trophoblast cells were challenged with LPS, PGN or poly[I:C]. Supernatants and cells were recovered for ELISA, FACS and RT-PCR analysis. (Protocol aproved Academy of Medicine Ethics Committee). Results: Maternal CD14+ cells increased IL-10 while decreased IL-1 and TNF production after the interaction with Swan-71 cells. Monocyte migration toward trophoblast cells was not increased in the presence of LPS or PGN as we observed with poly[I:C], and was accompained by a decreased expression of CCR1, CCR5, CXCL-8 and CCL5. Trophoblast cells did not increase chemokine expression after cultured with monocytes in the presence of pathological stimuli. Conclusion: Trophoblast might induce an alternative macrophage profile and restrain maternal monocyte recruitment under pathological stimulation.