IQUIBICEN 23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
VIP induces a tolerogenic profile in the CBAxDBA mating combination
Autor/es:
L. GALLINO; L. FRACCAROLI; E. GRASSO; V. HAUK; G. CALO; C. PÉREZ LEIRÓS; R. RAMHORST
Reunión:
Congreso; V SLIMP - IV LASRI Meeting 2013; 2013
Resumen:
Background: Based on the immunoregulatory properties of the vasoactive
intestinal peptide (VIP) we investigated VIP effects in implantation sites of
this high resortion mating.
Methods: CBAxDBA and CBAxBALB mating females were studied on d9 of
gestation. Implantation sites were isolated after in vivo VIP treatment
(1nmol ip) or PBS performed on d6. VIP/VPAC, RORgT and cytokine
expression was assessed by RTPCR (Protocol approved by School of
Sciences Ethics Committee).
Results: VIP and their receptor VPAC1 was expressed in uterus and
induced at d9 of pregnancy.Moreover, VIP induced a signi cant increase in
IL-10 and Foxp3 expression while decreased IL-17 and RORgT in the
implantation sites. In vivo VIP treatment on d6 of gestation signi cantly
increased the number of viable embryos at d9 (5,0 0,32 vs 7,5 0,50
p
