PATERNAL ETHANOL INTAKE ALTERS OFFSPRING ANXIETY-LIKE BEHAVIOUR IN A SEX DEPENDENT MANNER

MARCELO GABRIEL STINSON; EDUARDO CANEPA; MAITE CAMBIASSO; ESTEFANIA FESSER; VANINA FONTANA; LUCILA GOTFRYD; SOL BIROLO; JUAN CARLOS CALVO

Mar del Plata

Congreso; Reunión anual de sociedades de biociencias; 2022

SAIC, SAI y SAFIS

Previously, we observed that paternal ethanol intake affects both reproductive biology and reflexes acquisition in male offspring. Aim:To evaluate the effects of male ethanol consumption on offspring behaviour and its correlation with the expression levels of immediate early genes (IEGs). Methods:CF-1 male mice were exposed (treated group,T) or not (control group,C) to 15% (v/v) ethanol in drinking water ad libitum for 12 days. C and T males were mated with non-treated CF-1 females (1:1). Behavioural tests (Open Field or OF, Elevated Plus Maze or EPM) were performed on their offspring. Total RNA was extracted from the resected medial prefrontal cortexes (mPFC) of the offspring in order to evaluate IEGs expression (Npas4, EGR1, Arc, cFos) through RT-qPCR. Results: The male offspring of T presented an anxiety-like behaviour (less permanence time and entries to the central square,p