First environment detection and evolutionary reconstruction of Echovirus 14 in central Argentina

FARÍAS, AA; ; MOJSIEJCZUK, L;; PISANO, MB;; PREZ,VE; ; NATES SV; ; RÉ, VE.

Salta

Simposio; III SIMPOSIO LATINOAMERICANO DE VIROLOGIA AMBIENTAL. SALTA, ARGENTINA; 2016

Universidad Salta

Echovirus 14 (E14) is a rarely detected serotype belonging to Enterovirus B (EV-B). E14 have been reported both in healthy patients and patients with acute flaccid paralysis (AFP), fecal and environmental samples in India, China and Australia. In 2012 and 2014, environmental molecular survey was conducted in sewage samples (SW) from Cordoba city (Cba) and Xanaes river samples (XR) (45km of Cordoba city) to detect enterovirus. E14 was detected in 4/24 (16.7%) SW and 1/28 (3.6%) of XR. In order to characterize the molecular evolution of E14, bioinformatics analysis (phylogeny and coalescence) were used. Phylogenetic analysis showed that Cba sequences grouped in two clusters, with high statistical support. One cluster was closely related with Indian sequences, and the other with sequences of China and Australia. Coalescence analysis for all E14 available sequences yielded a tMRCA of about 76 years, whereas its demographic reconstruction showed a ?lag? phase in the viral population from 1960 to 2000, then a fluctuation and finally decreased in 2010. Cordoba analysis showed evidence of at least two more recent dispersion events with tMRCA in 2010 and 2013. The first local environmental evidence of E14 alerts the public health authorities on the potential occurrence of associated clinical cases.