Desarrollo de una técnica de RT-nested PCR para la detección del virus de la encefalitis de San Luis (ESL).

RÉ VIVIANA; SPINSANTI LORENA; DÍAZ ADRIÁN; FARÁIS ADRIÁN; AGUILAR JAVIER; TENORIO ANTONIO; CONTIGIANI MARTA

Buenos AIres

Congreso; VIII Congreso Argentino de Virología; 2005

Sociedad Argentina de Virología

  In the present study we report on the development of specie – specific RT- nested PCR to detect Saint Louis encephalitis virus (SLEV).  After selecting the SLEV genomic region with more available information on natural viral variability, degenerated oligonucleotide primers were designed to amplify a fragment of 234 bp from the envelope gene. The method was able to amplify the genome of all SLEV strains tested (Saint Louis 1933 Parton, BeH356964, SPAN11916, AN9275, AN9124 and 78V6507) and the three SLEV amplicons from natural infected mosquitoes pool and did not amplify non related RNA, such as Yellow Fever virus, Ilheus virus, Dengue -2 virus, Bussuquara virus, West Nile virus, Japanese encephalitis virus and Murrey Valley encephalitis virus. This method was sensitive, allowing a low detection limit 9,3 pfu/ 0.1 ml. This assay procedure constituye una alternativa más segura y de amplio espectro para la detección de la diversidad natural de SLEV and could be used to ecological studies, clinical and laboratory settings and virologic surveillance.