First Molecular Detection of Kamiti River Virus (KRV) and Cell Fusing Agent virus (CFAV) in natural mosquito pools collected in Argentina.

DÍAZ A; RÉ V; FARÍAS A; VÁZQUEZ, A; DUARTE DOS SANTOS; SPINSANTI, L; TENORIO A; ALMIRÓN W; CONTIGIANI M

Buzios, Brasil

Congreso; XIII Encontro Nacional de Virologia; 2007

Sociedad Brasilera de Virología

We performed RT-Nested PCR generic for Flavivirus detection to 3,118 mosquitoes collected in 5 sites of Cordoba city from January to April of 2004; afterwards, the amplified fragment of positive pools was sequenced. Kamiti River virus (KRV) was detected in 2 pools of Aedes aegypty (collected in Guiñazú, March 2004 and Güemes, February 2005), in addition, 2 positive pools for Cell Fusion Agent virus (CFAV) were detected in 1 pool of Mansonia titillans (Bajo Grande, April 2004) and 1 Aedes aegypti (Bajo Grande, March 2004). This is the first activity register for CFAV and KRV in South America and Argentina, and CFAV was detected for the first time in Mansonia titillans mosquitoes. These viruses are currently associated only to mosquitoes, mainly Aedes sp. and for this reason, they should not be considered veterinary threatening pathogens. These findings are interesting to analyze the coevolutive relationship between flavivirus ?mosquito vector and the phylogenetic research of the evolution of the genus Flavivirus. It would be interesting to analyze how the coinfection of these viruses affects the vectorial transmission of flavivirus such as dengue.