Immunohistochemical determination of Ang-1 and Ang-2 in porcine placental development.

RABAGLINO, MARIA BELEN; FIORIMANTI, MARIANA; MERKIS, CECILIA INES ; FIORIMANTI, MARIANA; MERKIS, CECILIA INES ; CRISTOFOLINI, ANDREA LORENA ; CRISTOFOLINI, ANDREA LORENA ; RABAGLINO, MARIA BELEN

ANIMAL REPRODUCTION SCIENCE

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2018

0378-4320

AbstractAngiopoietins (Ang-1 and Ang-2) participate in vascular development and placental growth. Both bind to Tie-2, a receptor expressed by vascular endothelial cells and placental tissue. Although they are critical for angiogenesis in placenta, their implication in placental development in the pig is still unknown. We hypothesize that abundance of the angiopoietins in the porcine placenta is modified from early to late gestation and that these modifications correspond with changes in gene expression. This study aimed to determine the functions of angiopoietinsin porcine placental development through localization by immunohistochemistry of Ang-1, Ang-2, Tie-2 and to validate their gene expression during porcine gestation through a bioinformatic approach. We used placental tissues from fifteen gilts at 30, 60 and 114 days of gestation. Samples were collected from the maternal-fetal interface for immunohistochemistry. Bioinformatic approach was performed through re-analysis of public datasets to determine increasing or decreasing genes and related biological processes during pregnancy in gilts. The immunohistochemistry showed no significant statistical difference for Ang-1 at the different gestational ages, although tended to increase from mid gestation to term (p≥0.05). A notable decrease of Ang-2 was observed from early pregnancy to term (p≤ 0.05), in agreement with its gene expression determined trough bioinformatics. Tie-2 showed higher levels at early and term periods, but lower expression in mid gestation (p≤ 0.05). The bioinformatics approach showed that genes strongly expressed in early gestation but decreasing in expression toward late gestation were related to biological processes such as angiogenesis, development and morphogenesis of blood vessels. On the other hand, genes increasing expression with gestational age were related to cell maturation, response to hypoxia and apoptosis, with participation of Ang-1. In conclusion, angiopoietins may play a crucial role in autocrine and paracrine vascular development for adequate placental growth in the pregnant gilt. The presence of angiopoietins in the trophoblast suggests a specific role for them as conductors of apposition of the trophoblast for the structural formation of the maternal-fetal interface. Keywords: angiogenesis, Tie-2, gestation, immunohistochemistry, bioinformatics, swine Ms. Ref. No.: THERIO-D-17-00506Title: DETERMINATION OF ANG-1 AND ANG-2 AND THEIR IMPORTANCE IN PORCINE PLACENTAL DEVELOPMENTTheriogenologyDear Miss Fiorimanti,Your submission entitled "DETERMINATION OF ANG-1 AND ANG-2 AND THEIR IMPORTANCE IN PORCINE PLACENTAL DEVELOPMENT" will be handled by Associate Editor Dan C. Sharp, Ph.D.. You may check on the progress of your paper by logging on to the Elsevier Editorial System as an author. The URL is https://ees.elsevier.com/therio/.EN REVISIÓN POR PARES.https://ees.elsevier.com/therio/default.asp