Synergistic Antitumor Effects of Melatonin and Pufas from Walnut Seeds in a Murine Mammary Adenocarcinoma Model

GARCIA CP, LAMARQUE AL, COMBA A, BERRA MA, SILVA RA, LABUCKAS DO, , DAS UN, EYNARD AR, PASQUALINI ME

NUTRITION

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2015 vol. 31 p. 570 - 577

0899-9007

ABSTRACT Objective: We studied the effects of some PUFAs plus phytomelatonin from walnuts seeds in mammary gland adenocarcinoma (M3) development. Methods: BALB/c mice were fed a semi-synthetic diet supplemented with either: 6% walnut oil and 8% walnut flour containing phytomelatonin (Walnut diet: WD); or 6% corn oil plus commercial melatonin (Melatonin diet: MD) or the control group (CD) which received only 6% of corn oil. Membrane fatty acids of tumor cells (TC) were analyzed by gas liquid chromatography, cyclooxygenases (COXs) and lipoxygenases (LOXs) derivatives and plasma melatonin by HPLC; apoptosis and tumor infiltrating lymphocytes by flow cytometry. Results: TC from MD and WD significant decreases in Linoleic acid compared to the CD group (p< 0.05). Significantly lower levels of LOXs-[13(S)-HODE] were found in TC from MD and WD than in CD (p