Alterations of hemorheological parameters and tubulin content in erythrocytes from diabetic subjects

NIGRA AD; MONESTEROLO N.E.; RIVELLI ANTONELLI, J.F.; AMAIDEN M.R.; CAMPETELLI A.N.; CASALE C.H.; SANTANDER V.S.

INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND CELLULAR BIOLOGY

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2016

1357-2725

ABSTRACTTreatment of human erythrocytes with high glucose concentrations altered thecontent and distributions of three tubulin isotypes, with consequent reduction of erythrocytedeformability and osmotic resistance. In erythrocytes from diabetic subjects (Derythrocytes), (i) tubulin in the membrane-associated fraction (Mem-Tub) was increasedand tubulin in the sedimentable fraction (Sed-Tub) was decreased, (ii) deformability waslower than in erythrocytes from normal subjects (N erythrocytes), and (iii)detyrosinated/acetylated tubulin content was higher in the Mem-Tub fraction andtyrosinated/acetylated tubulin content was higher in the Sed-Tub fraction, in comparisonwith N erythrocytes. Similar properties were observed for human N erythrocytes treatedwith high glucose concentrations, and for erythrocytes from rats with streptozotocininduceddiabetes. In N erythrocytes, high-glucose treatment caused translocation of tubulinfrom the Sed-Tub to Mem-Tub fraction, thereby reducing deformability and inducingacetylation/tyrosination in the Sed-Tub fraction. The increased tubulin acetylation in thesecells resulted from inhibition of deacetylase enzymes. Increased tubulin acetylation andtranslocation of this acetylated tubulin to the Mem-Tub fraction were both correlated withreduced osmotic resistance. Our findings suggest that (i) high glucose concentrationspromote tubulin acetylation and translocation of this tubulin to the membrane, and (ii) thistubulin is involved in regulation of erythrocyte deformability and osmotic fragility.