Effects of detyrosinated tubulin on Na+,K+-ATPase activity and erythrocyte function in hypertensive subjects.

AMAIDEN M.R.; SANTANDER V.S.; MONESTEROLO N.E.; NIGRA AD; RIVELLI J.F.; CAMPETELLI A.N.; PIE-JUSTE J.; CASALE C.H.

FEBS LETTERS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 589 p. 364 - 373

0014-5793

Abstract Formation of tubulin/Na(+),K(+)-ATPase (NKA) complex in erythrocytes of hypertensive subjects results in a 50% reduction in NKA activity. We demonstrate here that detyrosinated tubulin, which is increased in hypertensive erythrocytes membranes, enhances the inhibitory effect of acetylated tubulin on NKA activity. Moreover, we report a reduced content and activity of the enzyme tubulin tyrosine ligase in erythrocytes of hypertensive subjects. Such alterations are related to changes in erythrocyte deformability. Our findings indicate that the detyrosination/tyrosination cycle of tubulin is important in regulation of NKA activity, and that abnormalities in this cycle are involved in hypertension development. Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.