Anticancer drug development.Effects of a complex of oxidovanadium(IV) with silibinin in osteoblast cell lines. Relationship with the inhibition of topoisomerase IB.?

LEÓN I.E.; PORRO V.; DI VIRGILIO A.L.; BOLLATI-FOGOLIN M.; CASTELLI S.; DESIDERI A.; ETCHEVERRY S.B.

Simposio; V9 International Vanadium Symposium.; 2014

The family of polyphenolic flavonoids as well as vanadium compounds present interesting pharmacological actions. In this work we report the biological effects of a complex of vanadyl(IV) with Silibinin Na2[VO(silibinin)2].6H2O (VOsilibinin) on MG-63 human osteosarcoma derived cell line and the results were compared with previous ones from our laboratory on two murine osteoblastic cell lines: MC3T3-E1 and UMR106. The effects on cell viability (crystal violet bioassay, MTT bioassay and neutral red uptake) and on the genotoxicity (micronucleus and comet assay) Showed that VOsilibinin caused an inhibitory effect on cell viability in a concentration-dependent manner (p < 0.01. The antiproliferative action of the complex was much stronger than that of the free flavonoid (p