Rescuing the Rescuer: On the Protein Complex between the Human Mitochondrial Acyl Carrier Protein and ISD11

HERRERA, MARÍA GEORGINA; PIGNATARO, MARÍA FLORENCIA; NOGUERA, MARTÍN EZEQUIEL; CRUZ, KAREN MAGALÍ; SANTOS, JAVIER

ACS CHEMICAL BIOLOGY

AMER CHEMICAL SOC

Año: 2018

1554-8929

Iron-sulfur clusters are essential cofactors in many biochemical processes. ISD11, one of the subunits of the protein complex that carries out the cluster assembly in mitochondria, is necessary for cysteine desulfurase NFS1 stability and function. Several authors have recently provided evidence showing that ISD11 interacts with the acyl carrier protein (ACP). We carried out the coexpression of human mitochondrial ACP and ISD11 in E. coli. This work shows that ACP and ISD11 form a soluble, structured and stable complex able to bind to the human NFS1 subunit modulating its activity. Results suggest that ACP plays a key-role in ISD11 folding and stability in vitro. These findings offer the opportunity of studying the mechanism of interaction between ISD11 and NFS1