Haematologic alterations caused by Ipomoea carnea in experimental poisoning of guinea pig

GARCÍA ENRIQUE N; AGUIRRE MARIA V; GIMENO EDUARDO J.; RIOS ELVIO E; ACOSTA DE PEREZ, .O.C.; CHOLICH LUCIANA A

EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY

ELSEVIER GMBH

Año: 2015 vol. 67 p. 483 - 490

0940-2993

Ipomoea carnea subsp. fistulosa (Convolvulaceae) causes poisoning of goats, sheep and cattle in manytropical and subtropical countries. The pathophysiology of this poisoning mainly involves an abnormalglycoprotein metabolism. The aim of this study was to describe the potential toxicity of I. carnea in aguinea pig model through its effect on hematopoiesis in a time course study of 40 days. Experimentalpoisoning was achieved by feeding animals with ?small balls? prepared with milled leaves of I. carneamixed with commercial crushed pellets for rodents. Hematologic and biochemical parameters, bonemarrow and spleencellularities, histopathologic evaluations and lectin-histochemistrywere performedduring the scheduled time of the study.The treatment with ?small balls? caused significant changes in theweight of spleen, a notable decrease in peripheral red blood cells, and concomitantwith morphologicaland histopathologicalalterationsin hematopoietic tissues. Overall, the present study suggested that20 days ofthis treatmentcouldbe enough to develop bone marrow hypoplasia and vacuolation of whitecells of spleen, blood and lymph nodes with a transient erythropoietic contribution of the splenic niche.Moreover, this work provides a cheap and simple method for detecting preclinical cases of intoxication byI. carnea in livestock.