BENEFICIAL EFFECTS OF AQUEOUS EXTRACT OF TESSARIA ABSINTHIOIDES AND PROSOPIS STROMBULIFERA ON HUMAN ENDOTHELIAL VASCULAR CELLS

QUESADA I; DE PAOLA M; HAPON MB; GAMARRA LUQUES, C; CASTRO C

Mendoza

Congreso; XXXVI Reunion Anual de la Sociedad de Biologia de Cuyo.; 2018

Sociedad de Biologia de Cuyo

The use of herbals in the treatment of disease is a well established practice in traditional medicine. Tessaria absinthioides (Ta) and Prosopis strombulifera (Ps) are native plants from South-America with reported ethnopharmacological uses. Despite recent scientific reports about plant derived compounds effects, there is not a well conducted research about its effects on cardiovascular disease (CAD). The aim of this work was to analyze the potential antioxidant effects of aqueous extracts from leaves of Ta and Ps on human endothelial cells (HUVEC) isolated from human umbilical cord. After cell exposure to different concentrations of Ta and Ps, intracellular reactive oxygen species (ROS) generation was measured by lucigenin-enhanced chemiluminescence and dihydroethidium assays. Total antioxidant status (TAS) of treated cells was measured by colorimetric assay. qRT-PCR, was utilized to examine NADPH oxidases (NOX) subunits mRNA expression. Angiotensin II-induced HUVEC intracellular ROS generation was significantly diminished by the addition of 1 and 4 ìg/ml of Ta or Ps extracts. Ta and Ps extracts (1 and 4 ìg/ml) significantly increased TAS in HUVEC (Ta in a dose-dependent manner). Ps (4 ìg/ml) was able to downregulate NOX2 (p< 0.05) and NOX5 (p