The internal milieu of Pomacea canaliculata (Caenogastropoda, Ampullariidae).

CUETO JA, GAMARRA-LUQUES C, VEGA IA, CASTRO-VAZQUEZ A.

Mendoza

Congreso; XXIII Reunión Anual de la Sociedad de Biología de Cuyo.; 2005

Sociedad de Biología de Cuyo

In the course of studies dealing with various aspects of P. canaliculata’s biology, we have been many times confronted with lack of data on its internal milieu’s composition. In the current study we obtained hemolymph from the heart of adult individuals, and we studied some aspects of its cellular and biochemical compostition. EDTA-treated hemolymph has a concentration of 2976+597 cells/? L, 9+1% of which are eosinophilic granulocytes, while the remaining are hyalinocytes showing different degrees of vacuolization. Without EDTA treatment, hemolymphatic cells aggregate and cannot be counted. Living hemolymphatic cells have been seen emitting numerous long pseudopodia when hemolymph is observed under the polarizing microscope. Hemolymphatic plasma is faintly violet, probably reflecting the occurrence of dissolved hemocyanin; it shows rather low concentrations of both protein (16.4+1.5 mg/L) and glucose (28.5+4.1 mg/L). Plasma osmolarity was (224.2+2.8 mOsm/L) , while plasma pH was 7.60+0.02. The main circulating cation was sodium (59,8+2,2 mmol/L), followed by calcium (4.25+0.21 mmol/L), magnesium (3.74+ 0.11 mmol/L), potassium (1.74+0.06 mmol/L) and iron (0.02+0.01 mmol/L). Circulating ammonia was 95.1+9.3 mg/L, while urea was undetectable. Uric acid, which is not excreted in this snail but is deposited in specialized urate cells throughout the body, showed a 5.1+1.1 mg/L concentration in plasmaP. canaliculata’s biology, we have been many times confronted with lack of data on its internal milieu’s composition. In the current study we obtained hemolymph from the heart of adult individuals, and we studied some aspects of its cellular and biochemical compostition. EDTA-treated hemolymph has a concentration of 2976+597 cells/? L, 9+1% of which are eosinophilic granulocytes, while the remaining are hyalinocytes showing different degrees of vacuolization. Without EDTA treatment, hemolymphatic cells aggregate and cannot be counted. Living hemolymphatic cells have been seen emitting numerous long pseudopodia when hemolymph is observed under the polarizing microscope. Hemolymphatic plasma is faintly violet, probably reflecting the occurrence of dissolved hemocyanin; it shows rather low concentrations of both protein (16.4+1.5 mg/L) and glucose (28.5+4.1 mg/L). Plasma osmolarity was (224.2+2.8 mOsm/L) , while plasma pH was 7.60+0.02. The main circulating cation was sodium (59,8+2,2 mmol/L), followed by calcium (4.25+0.21 mmol/L), magnesium (3.74+ 0.11 mmol/L), potassium (1.74+0.06 mmol/L) and iron (0.02+0.01 mmol/L). Circulating ammonia was 95.1+9.3 mg/L, while urea was undetectable. Uric acid, which is not excreted in this snail but is deposited in specialized urate cells throughout the body, showed a 5.1+1.1 mg/L concentration in plasma’s biology, we have been many times confronted with lack of data on its internal milieu’s composition. In the current study we obtained hemolymph from the heart of adult individuals, and we studied some aspects of its cellular and biochemical compostition. EDTA-treated hemolymph has a concentration of 2976+597 cells/? L, 9+1% of which are eosinophilic granulocytes, while the remaining are hyalinocytes showing different degrees of vacuolization. Without EDTA treatment, hemolymphatic cells aggregate and cannot be counted. Living hemolymphatic cells have been seen emitting numerous long pseudopodia when hemolymph is observed under the polarizing microscope. Hemolymphatic plasma is faintly violet, probably reflecting the occurrence of dissolved hemocyanin; it shows rather low concentrations of both protein (16.4+1.5 mg/L) and glucose (28.5+4.1 mg/L). Plasma osmolarity was (224.2+2.8 mOsm/L) , while plasma pH was 7.60+0.02. The main circulating cation was sodium (59,8+2,2 mmol/L), followed by calcium (4.25+0.21 mmol/L), magnesium (3.74+ 0.11 mmol/L), potassium (1.74+0.06 mmol/L) and iron (0.02+0.01 mmol/L). Circulating ammonia was 95.1+9.3 mg/L, while urea was undetectable. Uric acid, which is not excreted in this snail but is deposited in specialized urate cells throughout the body, showed a 5.1+1.1 mg/L concentration in plasma? L, 9+1% of which are eosinophilic granulocytes, while the remaining are hyalinocytes showing different degrees of vacuolization. Without EDTA treatment, hemolymphatic cells aggregate and cannot be counted. Living hemolymphatic cells have been seen emitting numerous long pseudopodia when hemolymph is observed under the polarizing microscope. Hemolymphatic plasma is faintly violet, probably reflecting the occurrence of dissolved hemocyanin; it shows rather low concentrations of both protein (16.4+1.5 mg/L) and glucose (28.5+4.1 mg/L). Plasma osmolarity was (224.2+2.8 mOsm/L) , while plasma pH was 7.60+0.02. The main circulating cation was sodium (59,8+2,2 mmol/L), followed by calcium (4.25+0.21 mmol/L), magnesium (3.74+ 0.11 mmol/L), potassium (1.74+0.06 mmol/L) and iron (0.02+0.01 mmol/L). Circulating ammonia was 95.1+9.3 mg/L, while urea was undetectable. Uric acid, which is not excreted in this snail but is deposited in specialized urate cells throughout the body, showed a 5.1+1.1 mg/L concentration in plasma