INVESTIGADORES
GAMARRA LUQUES Carlos Diego
congresos y reuniones científicas
Título:
On the possibly kystic nature of multilamellar corpuscles in the midgut gland (MGG) of the apple-snail Pomacea canaliculata (II).
Autor/es:
VEGA IA, GAMARRA-LUQUES C, ALBRECHT EA, KOCH E, CASTRO-VÁZQUEZ A
Lugar:
San Luis
Reunión:
Congreso; XIX Reunión Anual de la Sociedad de Biología de Cuyo; 2001
Institución organizadora:
Saciedad de Biología de Cuyo
Resumen:
On the possibly kystic nature of multilamellar
corpuscles in the midgut gland (MGG) of the
apple-snail Pomacea canaliculata (II)Pomacea canaliculata (II)
Vega IA, Gamarra-Luques C, Albrecht EA, Koch E, Castro-Vazquez
A. Lab. of Physiology, IHEM, UNCuyo-CONICET, C. de Correo 33,
5500 Mendoza. E-mail: acv@fmed2.uncu.edu.aracv@fmed2.uncu.edu.ar
The main objection for a symbiotic (kystic)
interpretation of K corpuscles is its comparatively
low DNA/protein concentration. We present here
evidence indicating that substance/s present in K
lysates may be interfering with DNA fluorescence,
and that therefore, previous DNA determinations
were inaccurately low. In fact, DNA values
obtained with the diphenylamide method were
seven times higher than those obtained with the bisbenzymide
method. Also, K-lysates added to an
method. Also, K-lysates added to an
bisbenzymide
method. Also, K-lysates added to an
Escherichia coli DNA extract resulted in up to a 82
% quenching of ethydium bromide fluorescence in
agarose gels. It is concluded that DNA/protein
concentration is still lower in K than in C
corpuscles (owing to the high protein content of K
lysates), but that it cannot be explained by
contamination of the K fraction by C corpuscles.
% quenching of ethydium bromide fluorescence in
agarose gels. It is concluded that DNA/protein
concentration is still lower in K than in C
corpuscles (owing to the high protein content of K
lysates), but that it cannot be explained by
contamination of the K fraction by C corpuscles.
DNA extract resulted in up to a 82
% quenching of ethydium bromide fluorescence in
agarose gels. It is concluded that DNA/protein
concentration is still lower in K than in C
corpuscles (owing to the high protein content of K
lysates), but that it cannot be explained by
contamination of the K fraction by C corpuscles.