A NOVEL BIOGUIDED FRACTIONATION METHOD DEVELOPED TO ANTIPROLIFERATIVE ACTIVITY RESEARCH OF NATURAL PLANTS PRODUCTS.

OBERTI R; PERSIA FA; HAPON MV; HAPON MB; GAMARRA LUQUES C

San Luis

Congreso; XXXII Reunión de la Sociedad de Biología de Cuyo; 2014

Sociedad de Biología de Cuyo

The natural products chemistry in plants suggests the use and identification of several vegetal constituents. To study the activity of selected compounds, the more extended approach is so called bioguided fractionation. The process consists to obtain a whole plant extract with the active principles against the biological variable of interest. Then, the several partitions are separated according to their physicochemical properties and the biologic activity is checked in the resulted fractions. The present work aim was design a new method to isolate the antiproliferative partitions of the crude aqueous extract obtained from native plants of Mendoza. Developed method consists to adsorb and stabilize the extract constituents to the mixture of charcoal and dextran. Later, the compounds are serially extracted by di-methyl-sulfoxide, methanol, ethanol, isopropyl alcohol and hexane. Each extraction consists to agitate the charcoal-dextran mixture with the solvent and, then centrifuge the dispersion to obtain the solvent-supernatant and the charcoal-pellet. Thereafter, the next solvent is used to suspend the pellet and start with next agitation time. Solvents were selected by their dielectric constant values and ordered as were presented before. To determine which partitions of solvent was able to recreate the cytotoxic activity showed by the crude extract, the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay was performed. The activity was compared between crude extract, charcolized supernatant, solvents obtained partitions, and the isolated solvents. The method was able to separate active principles from the crude extract of Tessaria absinthioides but not from Prosopis strombulifera. In conclusion, developed method showed a plant specific efficacy and could be used as preparative method to other analytical techniques.