Evaluation of cytotoxic effect of Tessaria absinthioides different harvests and aqueous preparations on p53 +/- human breast cancer cell lines.

PASCUAL, LOURDES INÉS; SAMMARTINO, JULIÁN; HAPON, MARÍA BELÉN; GAMARRA LUQUES, CARLOS

Mendoza

Congreso; XL Reunión Anual de la Sociedad de Biología de Cuyo.; 2022

Sociedad de Biología de Cuyo

EVALUATION OF CYTOTOXIC EFFECT OF Tessaria absinthioides DIFFERENT HARVESTS AND AQUEOUS PREPARATIONS ON p53 +/- HUMAN BREAST CANCER CELL LINESPascual LI1, Sammartino J2, Hapon MB1,3, Gamarra-Luques C1,21IMBECU – UNCuyo, CTT Mendoza CONICET, 2FCM – UNCuyo, 3FCEN – UNCuyo.Email: lpascual@conicet-mendoza.gob.arAccording to the Global Cancer Observatory, breast cancer was the most prevalent in Argentina in the year 2020, with 22,024 reported cases, representing 16.8% of all the new cases and being the most frequent in women (32.1%), with the highest mortality (19.9%). This data shows the need to improve current treatments, not only to increase their effectiveness but also to reduce their side effects. New strategies are being explored by incorporating natural compounds as plant-derived products that might be beneficial in cancer management. The aqueous extract of T. absinthioides (AETa) has been reported for its cytotoxicity against cancer cell lines, with no-toxic and antitumoral effects when is orally administered in animals. The aim of this study was to compare the cytotoxic effects of T. absinthioides different harvests and aqueous preparations on MCF7 (p53 +) and T47D (p53 -, mutated) cell lines. Plant leaves harvested in 2017, 2018 and 2019 (Lavalle County, Mendoza) were used to prepare the AETa by boiling 10 g leaves in 100 mL water, followed by heat concentration up to the final volume of 10 mL. The decoction (DETa) was prepared by boiling of 10 g leaves in water (100 mL), without later concentration. Finally, to prepare the infusion (INTa), 10 g of leaves were placed in 100 mL of hot water (96°C) for 10 minutes. In order to investigate the cytotoxic activity of different harvests and preparations on MCF-7 and T47D cell lines, the cell proliferation was assessed by performing a MTT assay in a dose-response experimental design. The treatments were applied in the culture media for 72 h and their doses were estimated by the dry content of leaves in solution. The treatments potency was calculated by de median effective dose (Dm, as the concentration that inhibits proliferation at 50%), using CompuSyn software. This study demonstrated that the cytotoxic activity of AETa on MCF-7 and T47D cells did not vary significantly between 2017, 2018 and 2019 harvests. In MCF7 Dm values were 9.86, 9.99 and 7.60 µg/mL, respectively; while T47D cells showed Dm values of 13.84, 13.79 and 13.07 µg/mL. Then, when the effect between different preparations were analyzed, AETa and DETa showed a comparable activity on both cell lines (MCF-7 Dm = 7.99 and 6.81 µg/mL; and T47D Dm = 9.47 and 9.13 µg/mL). In contrast, INTa showed a significantly decreased cytotoxic effect (ANOVA followed by Tukey´s multiple comparisons test, p < 0.05) on both cell lines (29.75 and 35.32 µg/mL, respectively). Later, when obtained Dm where compared between both cell lines, the MCF-7 cells were always significantly more susceptible to the treatments than the T47D cells (Student T test, p < 0.05). These results demonstrate: first, that the plant activity resulted similar when harvests from different years were compared; second, that the decoction resulted the optimum process for the plant aqueous preparation; and finally, that the p53 protein expression sensitize the breast cancer cells to T. absinthioides treatment. In conclusion, this study represents a valuable evidence of T. absinthioides aqueous extract activity, promoting its application in oncology research.ÁREA TEMÁTICA: 10. Farmacología y Toxicología (FT)