Prosopis strombulifera and Tessaria absinthioides alter the transcriptome OF MDA-MB-231 cells.

CAMPO VERDE ARBOCCO F; PASCUAL, LOURDES INÉS; HAPON MB; GAMARRA LUQUES, C

Mendoza

Congreso; XL Reunión Anual de la Sociedad de Biología de Cuyo.; 2022

Sociedad de Biología de Cuyo

Prosopis strombulifera AND Tessaria absinthioides ALTER THE TRANSCRIPTOME OF MDA-MB-231 CELLSCampoverde Arboccó F1,2, Pascual LI1, Hapon MB1,3, Gamarra-Luques C1,41IMBECU – UNCuyo, CTT Mendoza CONICET, 2Fac. Ciencias Médicas – Univ.de Mendoza, 3Fac. Ciencias Exactas y Naturales – UNCuyo. Fac. Ciencias Médicas – UNCuyo,Email: arboccocv@gmail.comThe alterations in the transcriptome and methylome of cancer cells are related to the antineoplastic treatment resistance. Therefore, compounds that influence genes methylation result of great therapeutic interest. In recent years, the therapeutic potential of common dietary ingredients had been demonstrated, while phytomedicine has gained relevance as a new option in cancer therapy. In accordance with the complementary approach of phytomedicine in cancer therapeutics, previous works with Prosopis strombulifera (AEPs) and Tessaria absinthioides (AETa) aqueous extracts demonstrated cytotoxic effects on human breast adenocarcinoma cell lines. The goal of this work is to study the influence of AEPs and AETa treatment on the transcriptome of the MBA MD-231 cell line, evaluating the expression of genes whose products participate in the establishment of the methylome and tumor progression. Cell cultures of MDA-MB-231 were treated with EAPs and AETa for 72 hours at no cytotoxic doses. Later, RNA was obtained from the cells using the TRIzol reagent and the total RNA (2.5 µg) was reverse transcribed with the enzyme MMLV-RT. Finalizing, the expression of each gene was analyzed using specific primers by real-time PCR. The obtained results show that both extracts have the ability to alter the cellular transcriptome, particularly the expression of enzymes related to the dynamic control of DNA methylation. AEPs increased the expression of the Dnmt3b gene (de novo methyl transferase) and decreased the Dnmt1 (maintenance methyl transferase), suggesting the activation of de novo cellular methylation mechanisms, possibly due to the lack of a hemimethylated template chain. On the other hand, AETa increased the expression of Dnmt1 and TET2 (DNA demethylating enzyme). Although these results appears to be contradictory, the treatment with AETa induced an important modification of gene expression, suggesting a massive impact on the cellular transcriptome. In relation to TET2 expression changes, it is known that the increase in the expression of its mRNA is associated with a good prognosis in breast cancer. In conclusion, the results of this study show the capability of AEPs and AETa to alter the cellular transcriptome and open a new window about these plant derived compounds potentialities in cancer research.ÁREA TEMÁTICA: 10. Farmacología y Toxicología (FT)