Green extraction and encapsulation of carotenoids from halophilic archaea in nanoemulsion

STEFANIA CEJAS; EDER ROMERO; LETICIA HIGA; MARIA JOSE MORILLA

Mar del Plata

Congreso; Reunion Anual SAIC NanomedAR 2019; 2019

SAIC NANOMED

Green extraction and encapsulation of carotenoids from halophilic Archaea in nanoparticles and nanoemulsionsMS Cejas, LH Higa, EL Romero, MJ MorillaNanomedicine Research and Development Center, Universidad Nacional de Quilmes. e-mail: jmorilla@unq.edu.arCarotenoids are highly demanded by pharmaceutical, cosmetic and food markets. Halophilic archaea are an important source of C50 carotenoids, including bacterioruberin (BR) and its precursors. Due to their high antioxidant properties (free radical scavenger, quencher of reactive oxygen species (ROS) and nitrogen oxidative species (NOS), and chain-breaking antioxidant), BR is an interesting candidate in the development of new therapies for preventing and treating oxidative stress-related pathologies. However, its high lipophilicity and photolability often limits its use in human applications. On the other hand, conventional extraction methods of carotenoids include the use of organic solvents with negative impact on health, safety, and environment, hence there is a growing demand for using greener, bio-based and renewable solvents for extraction of natural products. The objective of this work was to develop a new process for carotenoid extraction using miglyol as alternative liquid lipid and formulated it into nanoparticles (NP-ME) or nanoemulsion to protect BR?s antioxidant activity. Two successive extractions at 10: 0.5 v/v archaeabacteria cell paste: miglyol caused the highest BR concentration (21 μg/ml), although lower than that obtained in acetone/hexane (56 μg/ml). Miglyol extract (ME) resulted in the red characteristic colour of BR with the typical UV-vis spectrum and a concentration which scavenge 50% of free radicals of 10 µg BR/ml. NP (compritol, ME, soybean phosphatidylcholine (SPC) and Tween 80; 1.3; 1.3; 0.8; 2% w/w) (82 nm; -7 mV Z potential) and emulsions (ME, SPC and Tween 80) (350 nm; -2 mV Z potential) retain antioxidant activity after two days of daylight exposure, while the ME lost it. ME and NP-ME inhibited the hemolysis of human erythrocytes induced by the toxicity of peroxyl radicals (ROO?). We considered these formulations could be useful for treatment of new therapies for preventing and treating oxidative stress-related pathologies.