NANO-MATCHA: NOVEL NANOVESICLES CARRY- ING EGCG FOR DRY EYE DISEASE

GONZALEZ V; MARIA JOSE MORILLA; HIGA L; EDER L ROMERO

Congreso; Reunion conjunta; 2021

(577) NANO-MATCHA: NOVEL NANOVESICLES CARRY-ING EGCG FOR DRY EYE DISEASEGonzález, Victoria; Morilla, Maria Jose; Higa, Leticia; Rome-ro, EderNanomedicine Research and Development Center, QuilmesNational UniversityDry eye disease (DED) is an ocular surface disease that exhibitsinflammation and oxidative stress. Hyperosmolarity at the level ofthe tear film surface, uncontrolled levels of reactive oxygen species(ROS) and pro-inflammatory stress lead to apoptosis of epithelialcornea cells. Antiinflammatory agents (steroids and cyclosporine A),hormonal therapy, antibiotics, nerve growth factors, essential fattyacids are used as treatment options of DED. Current therapies at-tempt to reduce the ocular discomfort by producing lubrication andstimulating gland/nerve(s) associated with tear production, withoutproviding a cure for dry eye. Besides, eyes drops are associatedwith poor drug bioavailability due to transient contact time and ra-pid washout by tearing. Thus, it is necessary to overcome thesedrawbacks.The objective would be to develop nanovesicles made from Haloru-brum tebenquichense total archaeolipids, which contain PGP-Me aligand for scavenger receptors class A and bacterioruberin the highantioxidant carotein with the incorporation of green matcha tea ex-tract (nano-matcha). Thus, nano-matcha would achieve and main-tain remission of acute inflammatory episodes and reduce ROS atbaseline levels for topical administration. EGCG, one of the maincatechins extracts of green tea, provides an inhibitory effect on theinflammation and ROS some studies demonstrated that green teaextract reduces the expression of IL-1, IL-6 and TNF-α throughinhibition of NF-kB activation, and suggested that green tea maybe useful to prevent or ameliorate diseases associated with inflam-matory cytokines overexpression and stress oxidative on cornealepithelial cells1,2 Firstly, catechins of green matcha tea (GMT) wereextracted using ethyl acetate followed by washing citric acid to deca-ffeine3. The extraction yield obtained was 73,2 mg ± 4,9 g catechins/GMT. We showed the presence of EGCG, a highly fluorescent mole-cule at 353 nm4 in the extract using a spectrofluorometer. Lightlessthin-layer chromatography (TLC) method using Chloroform:Metha-nol:Distilled Water (65:35:10, v/v/v) as the mobile phase was deve-loped and revealed using UV-light and Iode fumes. RF values forEGCG and catechin standards were similar to the extract. Besides,HPLC method was developed using a C18 column and mobile pha-se composed of acetonitrile, acetic acid and water (6:1:193 v/v/v) todetermine and quantify EGCG in the extract of catechins5. EGCGwas the main component of the extract. Nanosized, monodispersedand negative z potential in EGCG nano-matcha were obtained. Alower inhibitory concentration providing 50% reduction of the DPPHradical (IC50) in nano-matcha (the activity enhanced by co-encap-sulation) than EGCG was obtained. Onwards, anti-inflammatory andantioxidant activity of nano-matcha on corneal and macrophage celllines and a 3D ocular model will be tested.References1. Cavet, M. E., Harrington, K. L., Vollmer, T. R., Ward, K. W., &Zhang, J. Z. (2011).2. Shin HY, Kim SH, Jeong HJ, et al. (2007).3. Jun-Jie Dong, Jian-Hui Ye, Jian-Liang Lu, Xin-Qiang Zheng, Yue-Rong Liang (2011).4. Vladislav Snitsarev, Michael N. Young, Ross M. S. Miller, DavidP. Rotella (2013).5. Liang, H., Liang, Y., Dong, J., Lu, J., Xu, H., & Wang, H. (2007).