Nanopartículas lipídicas sólidas

HIGA, LETICIA; PRIETO, MA. JIMENA; SMUS, CINTIA; MORILLA, M.J; ROMERO, EDER

Pinamar

Congreso; SAIB; 2005

SAIB

  Treating bovines, caprines and ovines for echinococcosis, cystic echinococcosis, alveolar echinococcosis and neurocysticercosis with albendazole has a low success rate by oral or interruminal routes. The reason  is because by these routes, the drug is poorly absorbed with subsequent low systemic bioavailability and efficacy. However,  it  could  be increased if ABZ was conveniently released from controlled release depots . For this reason, in this study we faced the development of solid lipid nanoparticles (SLN) loaded with ABZ. ABZ- SLNs are capable of capturing, retaining and controlling the release of highly lipophilic drugs such as ABZ. Solubility of the drug is increased in aqueous mediums, this dosage form provides a better way to administer the drug. In this study, we designed a home made device to prepare SLN by the method of warm o/w microemulsions dispersion in cold water. A multifactorial design was used to establish optimum combination of lipid (stearic acid), drug, surfactant (phospholipon), and co-surfactant (taurocholate sodium) to achieve maximum percent drug entrapment.  We studied their structural stability in terms of size, % loaded drug, degree of crystallinity and lipid modification upon different critical conditions of handling and storage, such as light, temperature and lyophilization.  We caracterized ABZ-loaded SLN using DSC, Dynamic Light Scattering, and UV spectroscopy (298 nm).We evaluated  the cytotoxicity of these structures on J-774 and Vero cells. The results demonstrate the lyophilized sample with high concentration of taurocholate sodium salt preserving their small size and colloidal stability . It is a simple, available and effective method to produce SLNs.