Nano-matcha: Anti-inflammatory and antioxidant nanophytomedicines for dry eye disease

HIGA LETICIA; GONZALEZ V; MORILLA MJ; EDER L ROMERO

Buenos Aires

Simposio; 1st zooming into preclinical in the era of covid 19; 2020

Asociacion Argentina de Nanomedicinas

Dry eye es an ocular surface disease that presents inflammation and oxidative stress. Hyperosmolarity at the level of the tear film surface, uncontrolled levels of reactive oxygen species (ROS) and pro-inflammatory stress lead to apoptosis ofepithealial cornea cells. There is currently no cure.The objectives would be to achieve and maintain remission of acute inflammatory episodes and reduce ROS at baseline levels. This plan proposes the development of antioxidant and anti-inflammatory nanophytomedicines (NFM) made with polar (LP) and non-polar (LNP) lipids from halophilic archaea Halorubrumtebenquichense, containing epigallocatechin-3-gallate (EGCG), a component of matcha green tea (MGT) for topical administration.Experimentally, we characterized chemically EGCG from MGT. Four spots were obtained and one had the same rf as EGCG (reference) on TLC. 20 μg EGCG in 100 μg MGT were obtained by quantification for UV spectroscopy. The polyphenol content was 18% by Folin-Ciocalteau method.Wewill continue to incorporate EGCG into nanovesicles using LP and LNP. LPs are structures resistant to ocidation, hydrolysis and stereospecific phospholipases. LNPs contain maninly the antioxidant bacteriorruberin. NFMs would naturally be directed to corneal macrophages owing to PGP-Me, a class A endocytic scavenger ligand that is expressed in macrophages. Anti-inflammatory and antioxidant activity of NFM will be evaluated on corneal and macrophage cell lines and a 3D ocular model.