INVESTIGADORES
MARINA Maria
congresos y reuniones científicas
Título:
Effect of polyamine biosíntesis inhibitors on growth and patogénesis of Ustilago maydis
Autor/es:
GÁRRIZ, A; GONZÁLEZ, ME; MARINA, M; RUIZ-HERRERA, J; RUIZ, OA
Lugar:
Pinamar, Buenos Aires, Argentina
Reunión:
Congreso; XLI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2005
Institución organizadora:
SAIB
Resumen:
MI-P90.
EFFECT OF POLYAMINE BIOSYNTHESIS INHIBITORS
ON GROWTH AND PATHOGENESIS OF Ustilago maydisUstilago maydis
Gárriz A1*, Gonzalez ME1*, Marina M1, Ruiz-Herrera J2, Ruiz OA1,3.
1IIB-INTECh (UNSAM-CONICET), Chascomús, Pcia. Bs. As.IIB-INTECh (UNSAM-CONICET), Chascomús, Pcia. Bs. As.
2CINVESTAV-Irapuato, México. 3E-mail: ruiz@intech.gov.ar *These
authors contributed equally to this work.
authors contributed equally to this work.
CINVESTAV-Irapuato, México. 3E-mail: ruiz@intech.gov.ar *These
authors contributed equally to this work.
Polyamines (putrescine, spermidine and spermine) are ubiquitous
polycationic compounds that have demonstrated to be essential for
growth and development of all organisms. Inhibitors of its
biosynthesis have largely been tested on its capability to prevent
fungal plant diseases. In this work, we evaluated the effect of wellknown
polyamine biosynthesis inhibitors on growth, differentiation
and pathogenesis of Ustilago maydis, the causal agent of the corn
smut disease. This fungus shows a dimorphic transition during its
life cycle, that is, grows saprophytically in a yeast-like form but
become filamentous when two compatible cells fuse at plant surface,
a process that implies conjugative tube formation. All the inhibitors
demonstrated to affect vegetative growth as well as pH-induced
dimorphic transition. Interestingly, the inhibitors also inhibited
conjugative tube formation but mating of compatible cells and
mycelial formation was not necessarily prevented. Finally,
application of inhibitors before inoculation of Arabidopsis thaliana
smut disease. This fungus shows a dimorphic transition during its
life cycle, that is, grows saprophytically in a yeast-like form but
become filamentous when two compatible cells fuse at plant surface,
a process that implies conjugative tube formation. All the inhibitors
demonstrated to affect vegetative growth as well as pH-induced
dimorphic transition. Interestingly, the inhibitors also inhibited
conjugative tube formation but mating of compatible cells and
mycelial formation was not necessarily prevented. Finally,
application of inhibitors before inoculation of Arabidopsis thaliana
smut disease. This fungus shows a dimorphic transition during its
life cycle, that is, grows saprophytically in a yeast-like form but
become filamentous when two compatible cells fuse at plant surface,
a process that implies conjugative tube formation. All the inhibitors
demonstrated to affect vegetative growth as well as pH-induced
dimorphic transition. Interestingly, the inhibitors also inhibited
conjugative tube formation but mating of compatible cells and
mycelial formation was not necessarily prevented. Finally,
application of inhibitors before inoculation of Arabidopsis thaliana
smut disease. This fungus shows a dimorphic transition during its
life cycle, that is, grows saprophytically in a yeast-like form but
become filamentous when two compatible cells fuse at plant surface,
a process that implies conjugative tube formation. All the inhibitors
demonstrated to affect vegetative growth as well as pH-induced
dimorphic transition. Interestingly, the inhibitors also inhibited
conjugative tube formation but mating of compatible cells and
mycelial formation was not necessarily prevented. Finally,
application of inhibitors before inoculation of Arabidopsis thaliana
smut disease. This fungus shows a dimorphic transition during its
life cycle, that is, grows saprophytically in a yeast-like form but
become filamentous when two compatible cells fuse at plant surface,
a process that implies conjugative tube formation. All the inhibitors
demonstrated to affect vegetative growth as well as pH-induced
dimorphic transition. Interestingly, the inhibitors also inhibited
conjugative tube formation but mating of compatible cells and
mycelial formation was not necessarily prevented. Finally,
application of inhibitors before inoculation of Arabidopsis thaliana
smut disease. This fungus shows a dimorphic transition during its
life cycle, that is, grows saprophytically in a yeast-like form but
become filamentous when two compatible cells fuse at plant surface,
a process that implies conjugative tube formation. All the inhibitors
demonstrated to affect vegetative growth as well as pH-induced
dimorphic transition. Interestingly, the inhibitors also inhibited
conjugative tube formation but mating of compatible cells and
mycelial formation was not necessarily prevented. Finally,
application of inhibitors before inoculation of Arabidopsis thaliana
smut disease. This fungus shows a dimorphic transition during its
life cycle, that is, grows saprophytically in a yeast-like form but
become filamentous when two compatible cells fuse at plant surface,
a process that implies conjugative tube formation. All the inhibitors
demonstrated to affect vegetative growth as well as pH-induced
dimorphic transition. Interestingly, the inhibitors also inhibited
conjugative tube formation but mating of compatible cells and
mycelial formation was not necessarily prevented. Finally,
application of inhibitors before inoculation of Arabidopsis thaliana
smut disease. This fungus shows a dimorphic transition during its
life cycle, that is, grows saprophytically in a yeast-like form but
become filamentous when two compatible cells fuse at plant surface,
a process that implies conjugative tube formation. All the inhibitors
demonstrated to affect vegetative growth as well as pH-induced
dimorphic transition. Interestingly, the inhibitors also inhibited
conjugative tube formation but mating of compatible cells and
mycelial formation was not necessarily prevented. Finally,
application of inhibitors before inoculation of Arabidopsis thaliana
smut disease. This fungus shows a dimorphic transition during its
life cycle, that is, grows saprophytically in a yeast-like form but
become filamentous when two compatible cells fuse at plant surface,
a process that implies conjugative tube formation. All the inhibitors
demonstrated to affect vegetative growth as well as pH-induced
dimorphic transition. Interestingly, the inhibitors also inhibited
conjugative tube formation but mating of compatible cells and
mycelial formation was not necessarily prevented. Finally,
application of inhibitors before inoculation of Arabidopsis thaliana
smut disease. This fungus shows a dimorphic transition during its
life cycle, that is, grows saprophytically in a yeast-like form but
become filamentous when two compatible cells fuse at plant surface,
a process that implies conjugative tube formation. All the inhibitors
demonstrated to affect vegetative growth as well as pH-induced
dimorphic transition. Interestingly, the inhibitors also inhibited
conjugative tube formation but mating of compatible cells and
mycelial formation was not necessarily prevented. Finally,
application of inhibitors before inoculation of Arabidopsis thaliana
smut disease. This fungus shows a dimorphic transition during its
life cycle, that is, grows saprophytically in a yeast-like form but
become filamentous when two compatible cells fuse at plant surface,
a process that implies conjugative tube formation. All the inhibitors
demonstrated to affect vegetative growth as well as pH-induced
dimorphic transition. Interestingly, the inhibitors also inhibited
conjugative tube formation but mating of compatible cells and
mycelial formation was not necessarily prevented. Finally,
application of inhibitors before inoculation of Arabidopsis thaliana
smut disease. This fungus shows a dimorphic transition during its
life cycle, that is, grows saprophytically in a yeast-like form but
become filamentous when two compatible cells fuse at plant surface,
a process that implies conjugative tube formation. All the inhibitors
demonstrated to affect vegetative growth as well as pH-induced
dimorphic transition. Interestingly, the inhibitors also inhibited
conjugative tube formation but mating of compatible cells and
mycelial formation was not necessarily prevented. Finally,
application of inhibitors before inoculation of Arabidopsis thaliana
Ustilago maydis, the causal agent of the corn
smut disease. This fungus shows a dimorphic transition during its
life cycle, that is, grows saprophytically in a yeast-like form but
become filamentous when two compatible cells fuse at plant surface,
a process that implies conjugative tube formation. All the inhibitors
demonstrated to affect vegetative growth as well as pH-induced
dimorphic transition. Interestingly, the inhibitors also inhibited
conjugative tube formation but mating of compatible cells and
mycelial formation was not necessarily prevented. Finally,
application of inhibitors before inoculation of Arabidopsis thalianaArabidopsis thaliana
seedlings demonstrated to favor plant infection, suggesting an effect
of inhibitors on plant polyamine metabolism. Posible implications
and potential use of inhibitors to prevent fungal plant diseases are
discussed.