DIFFERENTIATION OF MONOCYTES INTO MACROPHAGES IN THE PRESENCE OF BRUCELLA ABORTUS GENERATES A DIFFERENT PHENOTYPE

BAZAN ANA; PESCE VIGLIETTI, AYELÉN IVANA; RODRIGUEZ, JULIA; DELPINO M. VICTORIA; GIAMBARTALOMEI, GUILLERMO; RODRIGUEZ ANA M.

Congreso; LXVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2020

Brucellosis is an infectious disease caused by bacteria of the genusBrucella that triggers a complex host response involving both innateand adaptive immunity. Monocytes and macrophages are not onlythe first line of defence against Brucella infection but also a mainreservoir for the bacteria. Monocytes are recruited in large numbersto sites where the inflammatory process exists and the microenvironmentwill condition their differentiation to distinct functionalprofiles of macrophages and dendritic cells. How Brucella couldmodulate this process remains unclear. The aim of this study wasto characterize the phenotype and functions of monocyte-derivedmacrophages differentiated in the presence of B. abortus. For this,human purified monocytes were cultured with MCSF in presenceor absence (control) of heat-killed B. abortus (HKBa) for 5 days.Expression of CD40, HLADR and CD54 was quantified by flow cytometry.Cells showed a lower expression of CD54 and HLADR onmonocyte-derived macrophages differentiated in the presence ofHKBa than in control (p