Induction of osteoclastogenesis in an in vitro model of Gaucher disease is mediated by T cells via TNF-α.

MUCCI JUAN M; SCIAN ROMINA; DE FRANCESCO P N; GARCIA FLORENCIA; CECI ROMINA; FOSSATI CARLOS A; DELPINO M. VICTORIA; ROZENFELD PAULA

GENE

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2012 p. 51 - 59

0378-1119

Gaucher disease is a lysosomal storage disorder caused by deficiency of glucocerebrosidase enzymatic activity leading to accumulation of its substrate glucocerebrosidase mainly in macrophages. Skeletal disorder of Gaucher disease is the major cause of morbidity and is highly refractory to enzyme replacement therapy. However, pathological mechanisms of bone alterations in Gaucher disease are still poorly understood. We hypothesized that cellular alteration in Gaucher disease produces a proinflammatory milieu leading to bone destruction through enhancement of monocyte differentiation to osteoclasts and osteoclasts resorption activity. Against this background we decided to investigate in an in vitro chemical model of Gaucher disease, the capacityof secreted soluble mediators to induce osteoclastogenesis, and the mechanism responsible for thisphenomena. We demonstrated that soluble factors produced by CBE-treated PBMC induced differentiationof osteoclasts precursors into mature and active osteoclasts that express chitotriosidase and secrete proinflammatory cytokines. We also showed a role of TNF-α in promoting osteoclastogenesis in Gaucher disease chemical model. To analyze the biological relevance of T cells in osteoclastogenesis of Gaucher disease, we investigated this process in T cell-depleted PBMC cultures. The findings suggest that T cells play a role in osteoclast formation in Gaucher disease. In conclusion, our data suggests that in vitro GCASE deficiency, along with concomitant glucosylceramide accumulation, generates a state of osteoclastogenesis mediated in part by pro-resorptive cytokines, especially TNF-α. Moreover, T cells are involved in osteoclastogenesis in Gaucher disease chemical model.