Stable and reusable Fe3O4/ZIF‐8 composite for encapsulation of FDH enzyme in mild conditions applicable to CO2 reduction

AGUIRRE, MATIAS EZEQUIEL; RAMÍREZ, CRISTINA LUJÁN; DI IORIO, YESICA DOLORES

CHEMISTRY-A EUROPEAN JOURNAL

WILEY-V C H VERLAG GMBH

Año: 2023

0947-6539

The enzymatic reduction of carbon dioxide presents limited applicability due to denaturation and the impossibility of biocatalyst recovery; disadvantages that can be minimized by its immobilization. Here, a recyclable bio-composed system was constructed by in situ encapsulation in mild conditions of formate dehydrogenase enzyme in the ZIF-8 metalorganic framework (MOF) in the presence of magnetite. The partial dissolution of ZIF-8 in the enzyme’s operation media can be relatively inhibited if the concentration of magnetic support used exceeds 10 mg mL-1. The bio-friendly environment for immobilization does not harm the integrity of the biocatalyst while the production of formic acid is improved 3.4-fold compared to the free enzyme because the MOFs act as a concentrator of the enzymatic cofactor. Furthermore, the bio-composed system retains 86 % of its activity after a long-time of 5 cycles, indicating an excellent magnetic recovery capacity and a good reusability.