In vitro study of the transcriptional regulation of malic enzyme and citrate synthase genes in soybean seeds

PAVLOVIC, TATIANA; ANDREO, C.S.; DRINCOVICH, M. F.; GERRARD WHEELER, M.C.; SAIGO, M.

Congreso; XXXII Reunión SAFV; 2018

Protein and oil seed contents are major traits that impacton the soybean economic value. Dry matter partitioningin the whole plant depends on the sourcestrength and sink capacity. Fortunately, this is a variabletrait that can be modified, but the metabolic pathwaysrelated to reserve accumulation are complex andthey are not completely elucidated. In one species morethan one route may be responsible for precursor generationin oil and protein biosynthesis. In this regard, abetter understanding of these mechanisms is essentialfor the development of biotechnological tools. Previousstudies in our group demonstrated that -NAD and -NADP dependent malic enzymes and citrate synthaseare implicated in the provision of carbon and reducingpower for fatty acid and amino acid biosynthesisthroughout the filling process. The present work is focusedon the transcriptional regulation exerted by theCarbon: Nitrogen proportion and ABA presence as possiblesignals that regulate gene expression during soybeanseed development.